Format

Send to

Choose Destination
Sci Rep. 2015 Aug 4;5:12714. doi: 10.1038/srep12714.

Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress.

Author information

1
Laboratory of Cancer Epigenetics, Faculty of Medicine, ULB, 1070 Brussels, Belgium.
2
CRUK Cambridge Institute, University of Cambridge, Cambridge CB2 0RE, United Kingdom.
3
ULB, Center for Diabetes Research, Faculty of Medicine, ULB, 1070 Brussels, Belgium.
4
Laboratory of Vaccinology and Mucosal Immunity, Faculty of Medicine, ULB, 1070 Brussels, Belgium.
5
Institute of Biochemistry, Stuttgart University, 70569 Stuttgart, Germany.

Abstract

The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.

PMID:
26239807
PMCID:
PMC4523844
DOI:
10.1038/srep12714
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center