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Sci Rep. 2015 Aug 4;5:12693. doi: 10.1038/srep12693.

Reduction of butyrate- and methane-producing microorganisms in patients with Irritable Bowel Syndrome.

Author information

1
Digestive System Research Unit, Vall d'Hebron Research Institute, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain.
2
Digestive Unit, University Hospital Vall d'Hebron, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain.
3
1] Digestive Unit, University Hospital Vall d'Hebron, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain [2] Centro de Investigacion Biomedica en Red en el Área tematica de Enfermedades Heptaticas y Digestivas, CIBERehd, Instituto de Salud Carlos III, Madrid, Spain.
4
1] Digestive System Research Unit, Vall d'Hebron Research Institute, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain [2] Digestive Unit, University Hospital Vall d'Hebron, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain [3] Centro de Investigacion Biomedica en Red en el Área tematica de Enfermedades Heptaticas y Digestivas, CIBERehd, Instituto de Salud Carlos III, Madrid, Spain.
5
1] Digestive System Research Unit, Vall d'Hebron Research Institute, Passeig Vall d'Hebron 119-129, Barcelona 08035, Spain [2] Centro de Investigacion Biomedica en Red en el Área tematica de Enfermedades Heptaticas y Digestivas, CIBERehd, Instituto de Salud Carlos III, Madrid, Spain.

Abstract

The pathophysiology of irritable bowel syndrome (IBS) remains unclear. Here we investigated the microbiome of a large cohort of patients to identify specific signatures for IBS subtypes. We examined the microbiome of 113 patients with IBS and 66 healthy controls. A subset of these participants provided two samples one month apart. We analyzed a total of 273 fecal samples, generating more than 20 million 16S rRNA sequences. In patients with IBS, a significantly lower microbial diversity was associated with a lower relative abundance of butyrate-producing bacteria (P = 0.002; q < 0.06), in particular in patients with IBS-D and IBS-M. IBS patients who did not receive any treatment harboured a lower abundance of Methanobacteria compared to healthy controls (P = 0.005; q = 0.05). Furthermore, significant correlations were observed between several bacterial taxa and sensation of flatulence and abdominal pain (P < 0.05). Altogether, our findings showed that IBS-M and IBS-D patients are characterized by a reduction of butyrate producing bacteria, known to improve intestinal barrier function, and a reduction of methane producing microorganisms a major mechanism of hydrogen disposal in the human colon, which could explain excess of abdominal gas in IBS.

PMID:
26239401
PMCID:
PMC4523847
DOI:
10.1038/srep12693
[Indexed for MEDLINE]
Free PMC Article
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