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Mech Dev. 2015 Nov;138 Pt 2:64-72. doi: 10.1016/j.mod.2015.07.006. Epub 2015 Jul 31.

Tetrapod axial evolution and developmental constraints; Empirical underpinning by a mouse model.

Author information

1
Department of Genetics and Evolution, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva, Switzerland.
2
Department of Genetics and Evolution, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva, Switzerland; School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland. Electronic address: denis.duboule@unige.ch.

Abstract

The tetrapod vertebral column has become increasingly complex during evolution as an adaptation to a terrestrial life. At the same time, the evolution of the vertebral formula became subject to developmental constraints acting on the size of the cervical and thoraco-lumbar regions. In the course of our studies concerning the evolution of Hox gene regulation, we produced a transgenic mouse model expressing fish Hox genes, which displayed a reduced number of thoraco-lumbar vertebrae and concurrent sacral homeotic transformations. Here, we analyze this mutant stock and conclude that the ancestral, pre-tetrapodial Hox code already possessed the capacity to induce vertebrae with sacral characteristics. This suggests that alterations in the interpretation of the Hox code may have participated to the evolution of this region in tetrapods, along with potential modifications of the HOX proteins themselves. With its reduced vertebral number, this mouse stock violates a previously described developmental constraint, which applies to the thoraco-lumbar region. The resulting offset between motor neuron morphology, vertebral patterning and the relative positioning of hind limbs illustrates that the precise orchestration of the Hox-clock in parallel with other ontogenetic pathways places constraints on the evolvability of the body plan.

KEYWORDS:

Developmental constraint; Hind limb; Homeotic transformation; Hox genes; Sacrum; Tetrapod evolution

PMID:
26238020
PMCID:
PMC4678112
DOI:
10.1016/j.mod.2015.07.006
[Indexed for MEDLINE]
Free PMC Article

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