Format

Send to

Choose Destination
ACS Nano. 2015 Aug 25;9(8):8554-61. doi: 10.1021/acsnano.5b03688. Epub 2015 Aug 4.

Post-Translational Modification of Bionanoparticles as a Modular Platform for Biosensor Assembly.

Author information

1
Department of Chemical and Biomolecular Engineering, University of Delaware , Newark, Delaware 19716, United States.

Abstract

Context driven biosensor assembly with modular targeting and detection moieties is gaining significant attentions. Although protein-based nanoparticles have emerged as an excellent platform for biosensor assembly, current strategies of decorating bionanoparticles with targeting and detection moieties often suffer from unfavorable spacing and orientation as well as bionanoparticle aggregation. Herein, we report a highly modular post-translational modification approach for biosensor assembly based on sortase A-mediated ligation. This approach enables the simultaneous modifications of the Bacillus stearothermophilus E2 nanoparticles with different functional moieties for antibody, enzyme, DNA aptamer, and dye decoration. The resulting easy-purification platform offers a high degree of targeting and detection modularity with signal amplification. This flexibility is demonstrated for the detection of both immobilized antigens and cancer cells.

KEYWORDS:

bionanoparticles; biosensor; easy purification; modularity; post-translational modification; signal amplification

PMID:
26235232
DOI:
10.1021/acsnano.5b03688
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center