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Lipids. 2015 Oct;50(10):1029-41. doi: 10.1007/s11745-015-4057-1. Epub 2015 Aug 2.

Modulation of the Expression of Components of the Stress Response by Dietary Arachidonic Acid in European Sea Bass (Dicentrarchus labrax) Larvae.

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  • 1Grupo de Investigación en Acuicultura (GIA), Parque Científico Tecnológico Marino de Taliarte, Universidad de Las Palmas de Gran Canaria (ULPGC), Muelle de Taliarte s/n, Telde, 35214, Las Palmas, Canary Islands, Spain. daniel.montero@ulpgc.es.
  • 2Department of Biotechnology and Life Sciences, University of Insubria, Via Dunant, 3-21100, Varese, Italy.
  • 3Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling, FK9-4LA, UK.
  • 4Grupo de Investigación en Acuicultura (GIA), Parque Científico Tecnológico Marino de Taliarte, Universidad de Las Palmas de Gran Canaria (ULPGC), Muelle de Taliarte s/n, Telde, 35214, Las Palmas, Canary Islands, Spain.

Abstract

This study reports for the first time on European sea bass, Dicentrarchus labrax (L.), larvae, the effect of different levels of dietary arachidonic acid (ARA; 20:4n-6) on the expression of genes related to the fish stress response. Copies of mRNA from genes related to steroidogenesis [StAR (steroidogenic acute regulatory protein), c-Fos, and CYP11β (11β-hydroxylase gene)], glucocorticoid receptor complex [GR (glucocorticoid receptor) and HSP (heat shock proteins) 70 and 90) and antioxidative stress (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase] were quantified. Eighteen day-old larvae were fed for 14 days with three experimental diets with increasing levels of ARA (0.3, 0.6 and 1.2% d.w.) and similar levels of docosahexaenoic (22:6n-3) and eicosapentaenoic (20:5n-3) acids (5 and 3%, respectively). The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real time RT-PCR with the standard curve method (absolute quantification). Increase dietary levels of ARA induced a significantly (p < 0.05) down-regulation of genes related to cortisol synthesis, such as StAR and CYP11β and up-regulated genes related to glucocorticoid receptor complex, such as HSP70 and GR. No effects were observed on antioxidant enzymes gene expression. These results revealed the regulatory role of dietary ARA on the expression of stress-related genes in European sea bass larvae.

KEYWORDS:

Arachidonic acid; Dicentrarchus labrax; Glucocorticoid receptor; HSP; Stress

PMID:
26233819
DOI:
10.1007/s11745-015-4057-1
[PubMed - indexed for MEDLINE]
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