Format

Send to

Choose Destination
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jan 5;152:327-35. doi: 10.1016/j.saa.2015.07.090. Epub 2015 Jul 26.

5-Hydroxymethylfurfural modified rhodamine B dual-function derivative: Highly sensitive and selective optical detection of pH and Cu(2+).

Author information

1
Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China.
2
Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, Henan 475004, PR China. Electronic address: zhouyanmei@henu.edu.cn.
3
Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL 32611, United States.
4
Key Laboratory of Plant Stress Biology, Henan University, Kaifeng 475004, PR China.

Abstract

A dual-function optical chemosensor (RBF) was designed and easily synthesized by condensation reaction of 5-Hydroxymethylfurfural and rhodamine B hydrazide. RBF exhibited highly sensitive, highly selective and quick response to acidic pH. The fluorescence intensity of RBF exhibited a more than 41-fold increase within the pH range from 7.50 to 3.73 with a pKa value of 5.02, which could be successfully applied to monitor intracellular pH in living PC12 cells and HeLa cells. Additionally, the spectroscopy of UV-Vis and EDTA-adding experiments indicated that RBF was a highly selective and reversible colorimetric chemosensor for Cu(2+) in Tris-HCl (10mM, pH=7.2) aqueous buffer solution as well as other metal ions had no obvious interference. Moreover, RBF has been successfully applied to detect Cu(2+) in real water samples.

KEYWORDS:

Colorimetric; Cu(2+); Fluorescent; Live-cells imaging; Rhodamine B; pH

PMID:
26232576
DOI:
10.1016/j.saa.2015.07.090
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center