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J Biol Chem. 2015 Sep 18;290(38):22931-8. doi: 10.1074/jbc.R115.675942. Epub 2015 Jul 31.

DNA End Resection: Nucleases Team Up with the Right Partners to Initiate Homologous Recombination.

Author information

1
From the Institute of Molecular Cancer Research, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland cejka@imcr.uzh.ch.

Abstract

The repair of DNA double-strand breaks by homologous recombination commences by nucleolytic degradation of the 5'-terminated strand of the DNA break. This leads to the formation of 3'-tailed DNA, which serves as a substrate for the strand exchange protein Rad51. The nucleoprotein filament then invades homologous DNA to drive template-directed repair. In this review, I discuss mainly the mechanisms of DNA end resection in Saccharomyces cerevisiae, which includes short-range resection by Mre11-Rad50-Xrs2 and Sae2, as well as processive long-range resection by Sgs1-Dna2 or Exo1 pathways. Resection mechanisms are highly conserved between yeast and humans, and analogous machineries are found in prokaryotes as well.

KEYWORDS:

DNA end resection; DNA endonuclease; DNA helicase; DNA repair; nuclease; protein phosphorylation; recombination

PMID:
26231213
PMCID:
PMC4645618
DOI:
10.1074/jbc.R115.675942
[Indexed for MEDLINE]
Free PMC Article

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