Format

Send to

Choose Destination
Immunity. 2015 Aug 18;43(2):369-81. doi: 10.1016/j.immuni.2015.06.017. Epub 2015 Jul 28.

Distinct Murine Mucosal Langerhans Cell Subsets Develop from Pre-dendritic Cells and Monocytes.

Author information

1
Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University, Hadassah, Jerusalem 91120, Israel.
2
Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel.
3
General Surgery Department, Hadassah Hebrew University Medical Center, Jerusalem 91120, Israel.
4
Institute for Biomedical Engineering, Department of Cell Biology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany; Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 52074 Aachen, Germany.
5
Department of Periodontology, Faculty of Dental Medicine, Hadassah Medical Center, Jerusalem 91120, Israel.
6
Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University, Hadassah, Jerusalem 91120, Israel. Electronic address: avihaih@ekmd.huji.ac.il.

Abstract

Langerhans cells (LCs) populate the mucosal epithelium, a major entry portal for pathogens, yet their ontogeny remains unclear. We found that, in contrast to skin LCs originating from self-renewing radioresistant embryonic precursors, oral mucosal LCs derive from circulating radiosensitive precursors. Mucosal LCs can be segregated into CD103(+)CD11b(lo) (CD103(+)) and CD11b(+)CD103(-) (CD11b(+)) subsets. We further demonstrated that similar to non-lymphoid dendritic cells (DCs), CD103(+) LCs originate from pre-DCs, whereas CD11b(+) LCs differentiate from both pre-DCs and monocytic precursors. Despite this ontogenetic discrepancy between skin and mucosal LCs, the transcriptomic signature and immunological function of oral LCs highly resemble those of skin LCs but not DCs. These findings, along with the epithelial position, morphology, and expression of the LC-associated phenotype strongly suggest that oral mucosal LCs are genuine LCs. Collectively, in a tissue-dependent manner, murine LCs differentiate from at least three distinct precursors (embryonic, pre-DC, and monocytic) in steady state.

PMID:
26231115
DOI:
10.1016/j.immuni.2015.06.017
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center