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J Vet Med Sci. 2016 Jan;77(12):1701-3. doi: 10.1292/jvms.15-0112. Epub 2015 Jul 26.

Quantitative PCR detection of feline morbillivirus in cat urine samples.

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1
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture & Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.

Abstract

Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.

PMID:
26227478
PMCID:
PMC4710735
DOI:
10.1292/jvms.15-0112
[Indexed for MEDLINE]
Free PMC Article
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