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Ann Anat. 2015 Sep;201:65-78. doi: 10.1016/j.aanat.2015.06.001. Epub 2015 Jul 6.

Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry.

Author information

1
Instituto de Neurociencias de Castilla y León (INCYL), Laboratorio de Neuroanatomía de los Sistemas Peptidérgicos, c/Pintor Fernando Gallego 1, University of Salamanca, 37007 Salamanca, Spain. Electronic address: covenas@usal.es.
2
Neurobiología Celular y Química Molecular (Human Neuroanatomy Laboratory), Facultades de Medicina y Farmacia, Universidad de Castilla-La Mancha, Centro Regional de Investigaciones Biomédicas (CRIB), Avenida de Almansa 14, 02006 Albacete, Spain. Electronic address: Joaquin.GFuentes@uclm.es.
3
Servicio de Anatomía Patológica, Hospital Virgen del Rocío, Avenida Manuel Siurot s/n., 41013 Sevilla, Spain. Electronic address: eloy.rivas.sspa@juntadeandalucia.es.
4
Neurobiología Celular y Química Molecular (Human Neuroanatomy Laboratory), Facultades de Medicina y Farmacia, Universidad de Castilla-La Mancha, Centro Regional de Investigaciones Biomédicas (CRIB), Avenida de Almansa 14, 02006 Albacete, Spain; Kavli Institute for Systems Neuroscience, Center for Neural Computation, Norwegian University for Sciences and Technology (NTNU), Trondheim, Norway. Electronic address: MariaJose.Lagartos@uclm.es.
5
Instituto de Neurociencias de Castilla y León (INCYL), Laboratorio de Neuroanatomía de los Sistemas Peptidérgicos, c/Pintor Fernando Gallego 1, University of Salamanca, 37007 Salamanca, Spain; Institut pour le Développement de la Recherche en Pathologie Humaine et Thérapeutique (IDRPHT), 33400 Talence, France; Gemacbio, Lieu dit Berganton, 33127 Saint Jean d'Illac, France. Electronic address: mangasam@usal.es.
6
Institut pour le Développement de la Recherche en Pathologie Humaine et Thérapeutique (IDRPHT), 33400 Talence, France; Gemacbio, Lieu dit Berganton, 33127 Saint Jean d'Illac, France. Electronic address: mg.idrpht@wanadoo.fr.
7
Neurobiología Celular y Química Molecular (Human Neuroanatomy Laboratory), Facultades de Medicina y Farmacia, Universidad de Castilla-La Mancha, Centro Regional de Investigaciones Biomédicas (CRIB), Avenida de Almansa 14, 02006 Albacete, Spain. Electronic address: Mariamar.Arroyo@uclm.es.
8
Laboratorio de Neuroanatomía Humana, Facultad de Medicina de Albacete, Universidad de Castilla-La Mancha, Centro Regional de Investigaciones Biomédicas (CRIB), Avenida de Almansa 14, 02006 Albacete, Spain. Electronic address: Sandra.Cebada@alu.uclm.es.
9
Laboratorio de Neuroanatomía Humana, Facultad de Medicina de Albacete, Universidad de Castilla-La Mancha, Centro Regional de Investigaciones Biomédicas (CRIB), Avenida de Almansa 14, 02006 Albacete, Spain. Electronic address: Ricardo.Insausti@uclm.es.
10
Neurobiología Celular y Química Molecular (Human Neuroanatomy Laboratory), Facultades de Medicina y Farmacia, Universidad de Castilla-La Mancha, Centro Regional de Investigaciones Biomédicas (CRIB), Avenida de Almansa 14, 02006 Albacete, Spain. Electronic address: Pilar.Marcos@uclm.es.

Abstract

Vitamin C (Vit C) is an important antioxidant, exerts powerful neuroprotective brain effects and plays a role in neuronal development and maturation. Vit C is present in brain tissue at higher concentrations than in other organs, but its detailed distribution in brain is unknown. Immunohistochemical detection of this vitamin has been performed by using a highly specific antibody against Vit C. The aim of the present work was to analyze the distribution of Vit C in children's brainstems during postnatal development, comparing two groups of ages: younger and older than one year of life. In general, the same areas showing neurons with Vit C in young cases are also immunostained at older ages. The distribution of neurons containing Vit C was broader in the brainstems of older children, suggesting that brainstem neurons maintain or even increase their ability to retain Vit C along the life span. Immunohistochemical labeling revealed only cell bodies containing this vitamin, and no immunoreactive fibers were observed. The distribution pattern of Vit C in children's brainstems suggests a possible role of Vit C in brain homeostatic regulation. In addition, the constant presence of Vit C in neurons of locus coeruleus supports the important role of Vit C in noradrenaline synthesis, which seemed to be maintained along postnatal development.

KEYWORDS:

Brainstem; Children; Development; Mapping; Vitamin C

PMID:
26226232
DOI:
10.1016/j.aanat.2015.06.001
[Indexed for MEDLINE]

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