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Int J Mol Sci. 2015 Jul 27;16(8):17018-28. doi: 10.3390/ijms160817018.

miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2.

Author information

1
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. wellginger1987@hotmail.com.
2
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. liroy@163.com.
3
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. shangtaott@126.com.
4
The 17th Student Battalion, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. haohan2016@126.com.
5
The 17th Student Battalion, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. tangran2016@126.com.
6
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. pkgtmmu@163.com.
7
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. zhaojiqing392@sohu.com.
8
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. huiqinsun02@163.com.
9
Institute of Toxicology, School of Preventive Medicine, the Third Military Medical University, 30 Gaotanyan Street, Shapingba District, Chongqing 400038, China. zmzou@tmmu.edu.cn.

Abstract

BACKGROUND:

MiR-198 has been considered as an inhibitor of cell proliferation, invasion, migration and a promoter of apoptosis in most cancer cells, while its effect on non-cancer cells is poorly understood.

METHODS:

The effect of miR-198 transfection on HaCaT cell proliferation was firstly detected using Cell Count Kit-8 and the cell cycle progression was analyzed by flow cytometry. Using bioinformatics analyses and luciferase assay, a new target of miR-198 was searched and identified. Then, the effect of the new target gene of miR-198 on cell proliferation and cell cycle was also detected.

RESULTS:

Here we showed that miR-198 directly bound to the 3'-UTR of CCND2 mRNA, which was a key regulator in cell cycle progression. Overexpressed miR-198 repressed CCND2 expression at mRNA and protein levels and subsequently led to cell proliferation inhibition and cell cycle arrest in the G1 phase. Transfection ofSiCCND2 in HaCaT cells showed similar inhibitory effects on cell proliferation and cell cycle progression.

CONCLUSION:

In conclusion, we have identified that miR-198 inhibited HaCaT cell proliferation by directly targeting CCND2.

KEYWORDS:

HaCaT; cell proliferation; cyclin D2; miR-198

PMID:
26225959
PMCID:
PMC4581182
DOI:
10.3390/ijms160817018
[Indexed for MEDLINE]
Free PMC Article

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