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J Cell Sci. 2015 Sep 15;128(18):3466-77. doi: 10.1242/jcs.173013. Epub 2015 Jul 29.

The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis.

Author information

1
Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany.
2
Friedrich Miescher Laboratory of the Max Planck Society, Spemannstraße 39, Tübingen 72076, Germany wolfram.antonin@tuebingen.mpg.de.

Abstract

The metazoan nucleus breaks down and reassembles during each cell division. Upon mitotic exit, the successful reestablishment of an interphase nucleus requires the coordinated reorganization of chromatin and formation of a functional nuclear envelope. Here, we report that the histone demethylase LSD1 (also known as KDM1A) plays a crucial role in nuclear assembly at the end of mitosis. Downregulation of LSD1 in cells extends telophase and impairs nuclear pore complex assembly. In vitro, LSD1 demethylase activity is required for the recruitment of MEL28 (also known as ELYS and AHCTF1) and nuclear envelope precursor vesicles to chromatin, crucial steps in nuclear reassembly. Accordingly, the formation of a closed nuclear envelope and nuclear pore complex assembly are impaired upon depletion of LSD1 or inhibition of its activity. Our results identify histone demethylation by LSD1 as a new regulatory mechanism linking the chromatin state and nuclear envelope formation at the end of mitosis.

KEYWORDS:

ELYS; Histone modification; KDM1A; Lysine (K)-specific demethylase 1A; MEL28; Mitotic exit; NDC1; Nuclear envelope formation; Nuclear pore complex; POM121

PMID:
26224877
DOI:
10.1242/jcs.173013
[Indexed for MEDLINE]
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