Format

Send to

Choose Destination
Microsc Microanal. 2015 Aug;21(4):1017-1025. doi: 10.1017/S1431927615014324.

Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.

Author information

1
The National Resource for Automated Molecular Microscopy, La Jolla, California, 92037, USA.
2
New York Structural Biology Center, New York, New York, 10027, USA.
3
Scintillon Institute, San Diego, California, 92121, USA.
4
La Jolla Bioengineering Institute, San Diego, California, 92121, USA.
#
Contributed equally

Abstract

We describe a system for rapidly screening hundreds of nanoparticle samples using transmission electron microscopy (TEM). The system uses a liquid handling robot to place up to 96 individual samples onto a single standard TEM grid at separate locations. The grid is then transferred into the TEM and automated software is used to acquire multiscale images of each sample. The images are then analyzed to extract metrics on the size, shape, and morphology of the nanoparticles. The system has been used to characterize plasmonically active nanomaterials.

KEYWORDS:

TEM; automation; nanoparticles

PMID:
26223550
PMCID:
PMC4701052
DOI:
10.1017/S1431927615014324
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center