Actinotetraose L, a new tetrasaccharide derivative isolated from the mycelia of Amycolatopsis sp. HCa1

J Asian Nat Prod Res. 2015;17(11):1109-13. doi: 10.1080/10286020.2015.1052413. Epub 2015 Jul 28.

Abstract

A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]- β-D-glucopyranosyl-(1 → 2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1 → 2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed.

Keywords: Amycolatopsis; Pseudonocardiaceae; grasshopper; tetrasaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinomycetales / chemistry*
  • Animals
  • Grasshoppers / microbiology
  • Humans
  • Hypercalciuria
  • Immunosuppressive Agents / chemistry
  • Immunosuppressive Agents / isolation & purification*
  • Immunosuppressive Agents / pharmacology
  • Molecular Structure
  • Mycelium / chemistry
  • Nuclear Magnetic Resonance, Biomolecular
  • Oligosaccharides / chemistry
  • Oligosaccharides / isolation & purification*
  • Oligosaccharides / pharmacology
  • T-Lymphocytes / drug effects

Substances

  • Immunosuppressive Agents
  • Oligosaccharides
  • actinotetraose L

Supplementary concepts

  • Hypercalciuria, Absorptive, 1