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Nat Commun. 2015 Jul 28;6:7822. doi: 10.1038/ncomms8822.

The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology.

Author information

1
1] Johann-Friedrich-Blumenbach-Institut, GZMB, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany [2] Department Biologie, Entwicklungsbiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstraße 5, 91058 Erlangen, Germany.
2
Department Biologie, Entwicklungsbiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstraße 5, 91058 Erlangen, Germany.
3
Johann-Friedrich-Blumenbach-Institut, GZMB, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
4
Institut für Entwicklungsbiologie, Universität zu Köln, Zülpicher Straße 47b, 50674 Cologne, Germany.
5
Abteilung Bioinformatik, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Goldschmidtstr. 1, 37077 Göttingen, Germany.
6
1] Johann-Friedrich-Blumenbach-Institut, GZMB, Georg-August-Universität Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany [2] Abteilung Bioinformatik, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Goldschmidtstr. 1, 37077 Göttingen, Germany [3] Abteilung für Bioinformatik, Universitätsmedizin Göttingen, Goldschmidtstr. 1, 37077 Göttingen, Germany.
7
Institut für Mathematik und Informatik, Ernst Moritz Arndt Universität Greifswald, Walther-Rathenau-Straße 47, 17487 Greifswald, Germany.
8
1] TU Dresden, Medical Faculty Carl Gustav Carus, Medical Systems Biology, Fetscherstr. 74, 01307 Dresden, Germany [2] Eupheria Biotech GmbH, Dresden, Germany.
9
Abteilung für Genetik, Universität Rostock, Albert-Einsteinstr. 3, 18059 Rostock, Germany.
10
TU Dresden, Medical Faculty Carl Gustav Carus, Medical Systems Biology, Fetscherstr. 74, 01307 Dresden, Germany.

Abstract

Genetic screens are powerful tools to identify the genes required for a given biological process. However, for technical reasons, comprehensive screens have been restricted to very few model organisms. Therefore, although deep sequencing is revealing the genes of ever more insect species, the functional studies predominantly focus on candidate genes previously identified in Drosophila, which is biasing research towards conserved gene functions. RNAi screens in other organisms promise to reduce this bias. Here we present the results of the iBeetle screen, a large-scale, unbiased RNAi screen in the red flour beetle, Tribolium castaneum, which identifies gene functions in embryonic and postembryonic development, physiology and cell biology. The utility of Tribolium as a screening platform is demonstrated by the identification of genes involved in insect epithelial adhesion. This work transcends the restrictions of the candidate gene approach and opens fields of research not accessible in Drosophila.

PMID:
26215380
PMCID:
PMC4525174
DOI:
10.1038/ncomms8822
[Indexed for MEDLINE]
Free PMC Article

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