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Genet Mol Res. 2015 Jul 14;14(3):7811-20. doi: 10.4238/2015.July.14.7.

Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum.

Author information

1
Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
2
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
3
Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China zaiwenshan@sina.com.

Abstract

Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90- 3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato.

PMID:
26214462
DOI:
10.4238/2015.July.14.7
[Indexed for MEDLINE]
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