Send to

Choose Destination
Genet Mol Res. 2015 Jul 14;14(3):7811-20. doi: 10.4238/2015.July.14.7.

Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum.

Author information

Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China.
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
Wenzhou Vocational College of Science and Technology, Wenzhou, Zhejiang, China


Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90- 3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Fundacao de Pesquisas Cientificas de Ribeirao Preto
Loading ...
Support Center