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Genet Mol Res. 2015 Jul 3;14(3):7395-405. doi: 10.4238/2015.July.3.15.

rbcS SRS4 promoter from Glycine max and its expression activity in transgenic tobacco.

Author information

1
College of Life Sciences, Jilin Agricultural University, Changchun, China.
2
College of Agronomy, Jilin Agricultural University, Changchun, China.
3
Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China.

Abstract

The regulatory region of the ribulose-1,5-bisphosphate carboxylase small subunit gene SRS4 from soybean (Glycine max) was cloned using TAIL-PCR and general PCR, and named the rbcS promoter. The promoter was fused with the GUS gene and introduced into Nicotiana tabacum via Agrobacterium-mediated leaf disk transformation. In 4-week-old transgenic tobacco plants, the highest GUS expression levels were observed in the leaves, GUS activity was 7.13- and 7.40-fold higher in leaves than in stems and roots, respectively. Moreover, GUS activity was stimulated by light. In conclusion, spatial and light regulation of the soybean rbcS promoter was observed in N. tabacum, thus illustrating a leaf-specific and light-induced promoter.

PMID:
26214418
DOI:
10.4238/2015.July.3.15
[Indexed for MEDLINE]
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