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Stem Cell Reports. 2015 Aug 11;5(2):278-90. doi: 10.1016/j.stemcr.2015.06.005. Epub 2015 Jul 23.

Boundary Caps Give Rise to Neurogenic Stem Cells and Terminal Glia in the Skin.

Author information

1
Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France.
2
Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France; Sorbonne Universités, UPMC Université Paris 06, IFD, 4 Place Jussieu, 75252 Paris Cedex 05, France.
3
National Reference Centre "Rare Peripheral Neuropathies" Department of Neurology, Centre Hospitalier Universitaire de Limoges, 87042 Limoges, France.
4
Ecole Normale Supérieure, Institut de Biologie de l'ENS (IBENS), and INSERM U1024, and Centre National de la Recherche Scientifique (CNRS) UMR 8197, Paris 75005, France. Electronic address: patrick.charnay@ens.fr.

Abstract

While neurogenic stem cells have been identified in rodent and human skin, their manipulation and further characterization are hampered by a lack of specific markers. Here, we perform genetic tracing of the progeny of boundary cap (BC) cells, a neural-crest-derived cell population localized at peripheral nerve entry/exit points. We show that BC derivatives migrate along peripheral nerves to reach the skin, where they give rise to terminal glia associated with dermal nerve endings. Dermal BC derivatives also include cells that self-renew in sphere culture and have broad in vitro differentiation potential. Upon transplantation into adult mouse dorsal root ganglia, skin BC derivatives efficiently differentiate into various types of mature sensory neurons. Together, this work establishes the embryonic origin, pathway of migration, and in vivo neurogenic potential of a major component of skin stem-like cells. It provides genetic tools to study and manipulate this population of high interest for medical applications.

PMID:
26212662
PMCID:
PMC4618659
DOI:
10.1016/j.stemcr.2015.06.005
[Indexed for MEDLINE]
Free PMC Article

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