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Stem Cell Reports. 2015 Sep 8;5(3):365-77. doi: 10.1016/j.stemcr.2015.06.006. Epub 2015 Jul 23.

MicroRNA-153 Regulates the Acquisition of Gliogenic Competence by Neural Stem Cells.

Author information

1
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-Ku, Tokyo 160-8582, Japan.
2
Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4067, Australia.
3
Queensland Brain Institute, The University of Queensland, Brisbane, QLD 4067, Australia; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD 4067, Australia.
4
Department of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.
5
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-Ku, Tokyo 160-8582, Japan. Electronic address: shimazaki@keio.jp.
6
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi Shinjuku-Ku, Tokyo 160-8582, Japan. Electronic address: hidokano@a2.keio.jp.

Abstract

Mammalian neural stem/progenitor cells (NSPCs) sequentially generate neurons and glia during CNS development. Here we identified miRNA-153 (miR-153) as a modulator of the temporal regulation of NSPC differentiation. Overexpression (OE) of miR-153 delayed the onset of astrogliogenesis and maintained NSPCs in an undifferentiated state in vitro and in the developing cortex. The transcription factors nuclear factor I (NFI) A and B, essential regulators of the initiation of gliogenesis, were found to be targets of miR-153. Inhibition of miR-153 in early neurogenic NSPCs induced precocious gliogenesis, whereas NFIA/B overexpression rescued the anti-gliogenic phenotypes induced by miR-153 OE. Our results indicate that miR-mediated fine control of NFIA/B expression is important in the molecular networks that regulate the acquisition of gliogenic competence by NSPCs in the developing CNS.

PMID:
26212661
PMCID:
PMC4618452
DOI:
10.1016/j.stemcr.2015.06.006
[Indexed for MEDLINE]
Free PMC Article

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