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Mol Cell. 2015 Aug 6;59(3):502-11. doi: 10.1016/j.molcel.2015.06.022. Epub 2015 Jul 23.

An Interactive Database for the Assessment of Histone Antibody Specificity.

Author information

1
Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA. Electronic address: scott.rothbart@vai.org.
2
Center for Epigenetics, Van Andel Research Institute, Grand Rapids, MI 49503, USA.
3
Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
4
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA.
5
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
6
Laboratory of Chromatin Biology and Epigenetics, The Rockefeller University, New York, NY 10065, USA.
7
Department of Biology, Tufts University, Medford, MA 02155, USA.
8
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
9
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Electronic address: brian_strahl@med.unc.edu.

Abstract

Access to high-quality antibodies is a necessity for the study of histones and their posttranslational modifications (PTMs). Here we debut the Histone Antibody Specificity Database (http://www.histoneantibodies.com), an online and expanding resource cataloging the behavior of widely used, commercially available histone antibodies by peptide microarray. This interactive web portal provides a critical resource to the biological research community that routinely uses these antibodies as detection reagents for a wide range of applications.

PMID:
26212453
PMCID:
PMC4530063
DOI:
10.1016/j.molcel.2015.06.022
[Indexed for MEDLINE]
Free PMC Article

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