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Dev Cell. 2015 Aug 10;34(3):373-8. doi: 10.1016/j.devcel.2015.06.003. Epub 2015 Jul 23.

Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening.

Author information

1
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA. Electronic address: andylane@berkeley.edu.
2
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA.
3
Department of Cell & Tissue Biology, University of California at San Francisco, San Francisco, CA 94143-0512, USA.
4
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3200, USA. Electronic address: bheald@berkeley.edu.

Abstract

CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we generated guide libraries that label repetitive loci or a single chromosomal locus in Xenopus egg extracts and show that a complex library can target the E. coli genome at high frequency.

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PMID:
26212133
PMCID:
PMC4536113
DOI:
10.1016/j.devcel.2015.06.003
[Indexed for MEDLINE]
Free PMC Article

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