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PLoS One. 2015 Jul 24;10(7):e0132953. doi: 10.1371/journal.pone.0132953. eCollection 2015.

Cholangiocarcinoma: Correlation between Molecular Profiling and Imaging Phenotypes.

Author information

1
Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America.
2
Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America; Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America.
3
Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America.
4
Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America.
5
Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America.

Abstract

PURPOSE:

To investigate associations between imaging features of cholangiocarcinoma by visual assessment and texture analysis, which quantifies heterogeneity in tumor enhancement patterns, with molecular profiles based on hypoxia markers.

METHODS:

The institutional review board approved this HIPAA-compliant retrospective study of CT images of intrahepatic cholangiocarcinoma, obtained before surgery. Immunostaining for hypoxia markers (EGFR, VEGF, CD24, P53, MDM2, MRP-1, HIF-1α, CA-IX, and GLUT1) was performed on pre-treatment liver biopsies. Quantitative imaging phenotypes were determined by texture analysis with gray level co-occurrence matrixes. The correlations between quantitative imaging phenotypes, qualitative imaging features (measured by radiographic inspection alone), and expression levels of the hypoxia markers from the 25 tumors were assessed.

RESULTS:

Twenty-five patients were included with a median age of 62 years (range: 54-84). The median tumor size was 10.2 cm (range: 4-14), 10 (40%) were single tumors, and 90% were moderately differentiated. Positive immunostaining was recorded for VEGF in 67% of the cases, EGFR in 75%, and CD24 in 55%. On multiple linear regression analysis, quantitative imaging phenotypes correlated significantly with EGFR and VEGF expression levels (R2 = 0.4, p<0.05 and R2 = 0.2, p<0.05, respectively), while a trend was demonstrated with CD24 expression (R2 = 0.33, p = 0.1). Three qualitative imaging features correlated with VEGF and CD24 expression (P<0.05), however, none of the qualitative features correlated with the quantitative imaging phenotypes.

CONCLUSION:

Quantitative imaging phenotypes, as defined by texture analysis, correlated with expression of specific markers of hypoxia, regardless of conventional imaging features.

PMID:
26207380
PMCID:
PMC4514866
DOI:
10.1371/journal.pone.0132953
[Indexed for MEDLINE]
Free PMC Article

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