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Angew Chem Int Ed Engl. 2015 Aug 24;54(35):10216-9. doi: 10.1002/anie.201503215. Epub 2015 Jul 15.

SLAP: Small Labeling Pair for Single-Molecule Super-Resolution Imaging.

Author information

1
Institute of Biochemistry, Biocenter; Cluster of Excellence Frankfurt, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt/M. (Germany) http://www.biochem.uni-frankfurt.de http://www.smb.uni-frankfurt.de.
2
Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt/M. (Germany).
3
Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt/M. (Germany). heilemann@chemie.uni-frankfurt.de.
4
Institute of Biochemistry, Biocenter; Cluster of Excellence Frankfurt, Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt/M. (Germany) http://www.biochem.uni-frankfurt.de http://www.smb.uni-frankfurt.de. tampe@em.uni-frankfurt.de.

Abstract

Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. A sensitive and efficient modular labeling approach (SLAP) was developed on the basis of a synthetic small-molecule recognition unit (Ni-trisNTA) and the genetically encoded minimal protein His6-10 -tag. High-density protein tracing by SLAP was demonstrated. This technique allows super-resolution fluorescence imaging and fulfills the necessary sampling criteria for single-molecule localization-based imaging techniques. It avoids masking by large probes, for example, antibodies, and supplies sensitive, precise, and robust size analysis of protein clusters (nanodomains).

KEYWORDS:

bioorthogonal chemistry; chemical biology; proteins; single-molecule imaging; super-resolution microscopy

PMID:
26201868
DOI:
10.1002/anie.201503215
[Indexed for MEDLINE]

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