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Aust N Z J Obstet Gynaecol. 2015 Aug;55(4):379-83. doi: 10.1111/ajo.12373. Epub 2015 Jul 23.

Sperm DNA fragmentation abnormalities in men from couples with a history of recurrent miscarriage.

Author information

1
University of New South Wales, Sydney, NSW, Australia.
2
Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW, Australia.
3
St George Hospital, Kogarah, Sydney, NSW, Australia.
4
Royal Hospital for Women, Randwick, NSW, Australia.
5
IVFAustralia, Sydney, NSW, Australia.

Abstract

BACKGROUND:

Previous studies have described an association between sperm with DNA damage and a history of recurrent miscarriage (RM), although it is not clear whether there is benefit in screening for sperm DNA fragmentation and to what extent DNA fragmentation impacts upon RM.

AIMS:

To identify what proportion of couples experiencing RM are affected by DNA fragmentation abnormalities.

MATERIALS AND METHODS:

In this retrospective study, between 2008 and 2013, couples with a history of recurrent miscarriage (≥3 first trimester miscarriages) were investigated comprehensively for known causes (karyotype, uterine, antiphospholipid syndrome, thrombophilia) and also by semen analysis, including DNA fragmentation [sperm chromatin structure analysis (SCSA)]. Statistical analysis was performed on SPSS software with significance taken as P < 0.05.

RESULTS:

There were 108 couples with a median sperm DNA fragmentation index (DFI) of 9.50%. Normal levels were found in 70.5% of men (DFI < 15%), 23% had high levels (DFI 15-30%), and 6.5% had very high levels (DFI > 30%). Couples with otherwise unexplained recurrent miscarriage had significantly higher DFI than those with other causes identified on routine screening (P = 0.012).

CONCLUSIONS:

In couples experiencing RM, 30% (32/108) of men had sperm with high levels of DNA fragmentation (DFI > 15%). This may be a contributing factor to the clinical syndrome of RM, and future clinical trials of therapies for these couples are warranted.

KEYWORDS:

DNA Fragmentation; DNA damage; recurrent miscarriage; recurrent spontaneous abortion; sperm

PMID:
26201831
DOI:
10.1111/ajo.12373
[Indexed for MEDLINE]

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