Format

Send to

Choose Destination
G3 (Bethesda). 2015 Jul 21;5(9):1919-24. doi: 10.1534/g3.115.019364.

Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells.

Author information

1
Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 stephanie_mohr@hms.harvard.edu.
2
Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
3
Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109.
4
Drosophila RNAi Screening Center, Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 Howard Hughes Medical Institute, Boston, Massachusetts 02115.

Abstract

RNA binding proteins (RBPs) are involved in many cellular functions. To facilitate functional characterization of RBPs, we generated an RNA interference (RNAi) library for Drosophila cell-based screens comprising reagents targeting known or putative RBPs. To test the quality of the library and provide a baseline analysis of the effects of the RNAi reagents on viability, we screened the library using a total ATP assay and high-throughput imaging in Drosophila S2R+ cultured cells. The results are consistent with production of a high-quality library that will be useful for functional genomics studies using other assays. Altogether, we provide resources in the form of an initial curated list of Drosophila RBPs; an RNAi screening library we expect to be used with additional assays that address more specific biological questions; and total ATP and image data useful for comparison of those additional assay results with fundamental information such as effects of a given reagent in the library on cell viability. Importantly, we make the baseline data, including more than 200,000 images, easily accessible online.

KEYWORDS:

Drosophila; RNA binding protein; RNAi; data sharing; high-throughput screen

PMID:
26199285
PMCID:
PMC4555228
DOI:
10.1534/g3.115.019364
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center