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BMC Complement Altern Med. 2015 Jul 22;15:246. doi: 10.1186/s12906-015-0773-6.

Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress.

Author information

1
Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran. armoghadm@hotmail.com.
2
Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran. soheyl_7@yahoo.com.
3
Young Researchers and Elite club, Tabriz Branch, Islamic Azad University, Tabriz, Iran, Shiraz, Iran. dr.namvran@gmail.com.
4
Faculty of Veterinary Medicine, Tehran University, Tehran, Iran. Mina.Yazdi@gmail.com.
5
Faculty of Medicine, Tehran Branch, Islamic Azad University, Tehran, Iran. f.bonyadi86@gmail.com.
6
Department of Pathobiology, Tabriz Branch, Islamic Azad University, Islamic Azad University, Tabriz, Iran. daryoushmohajeri@yahoo.com.
7
Department of Biochemistry, Ardabil University of Medical Science, Ardabil, Iran. m.mazani@arums.ac.ir.
8
Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada. hassan.marzban@med.umanitoba.ca.
9
Department of Clinical and Experimental Medicine (IKE), Division of Cell Biology, and Integrative Regenerative Medicine Center (IGEN), Linköping University, Linköping, Sweden. marek.los@liu.se.
10
Department of Pathology, Pomeranian Medical University, Szczecin, Poland. marek.los@liu.se.
11
ENT Department, School of Medicine, Medical University of Silesia, Katowice, Poland. marek.los@liu.se.
12
Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada. saeid.Ghavami@gmail.com.
13
The Children Hospital Research Institute of Manitoba, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada. saeid.Ghavami@gmail.com.
14
Health Policy Research Centre, Shiraz Medical University, Shiraz, Iran. saeid.Ghavami@gmail.com.

Abstract

BACKGROUND:

Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity.

METHODS:

All experiments were performed on male Wistar albino rats that were randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two and three received turmeric extract (100, 200 mg/kg respectively) orally for 30 days, group four received single dose, of MTX IP at day 30, groups five and six received turmeric extract 100 and 200 mg/kg orally respectively for 30 days and single dose of methoterxate IP (20 mg/kg) at day 30. Four days after MTX injection animals were sacrificed and evaluated. Blood ALT and AST (indicators of hepatocyte injury), ALP and bilirubin (markers of biliary function), albumin (reflect liver synthetic function) as well as the plasma TAS concentration (antioxidant defenses) were determined. The cellular antioxidant defense activities were examined in liver tissue samples using SOD, CAT, and GSH-Px for the oxidative stress, and MDA for lipid peroxidation. In addition, liver damage was evaluated histopathologically.

RESULTS:

MTX significantly induced liver damage (P<0.05) and decreased its antioxidant capacity, while turmeric was hepatoprotective. Liver tissue microscopic evaluation showed that MTX treatment induced severe centrilobular and periportal degeneration, hyperemia of portal vein, increased artery inflammatory cells infiltration and necrosis, while all of histopathological changes were attenuated by turmeric (200 mg/kg).

CONCLUSION:

Turmeric extract can successfully attenuate MTX-hepatotoxicity. The effect is partly mediated through extract's antinflammatory activity.

PMID:
26199067
PMCID:
PMC4511036
DOI:
10.1186/s12906-015-0773-6
[Indexed for MEDLINE]
Free PMC Article

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