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J Neuroimmunol. 2015 Aug 15;285:169-79. doi: 10.1016/j.jneuroim.2015.06.007. Epub 2015 Jun 20.

Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice.

Author information

1
Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics and PolicySchool of PharmacyUniversity of Southern California1985 Zonal Avenue, Los Angeles, CA, 90033, United States. Electronic address: asatryan@usc.edu.
2
Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90033, United States.
3
Titus Family Department of Clinical Pharmacy and Pharmaceutical Economics and PolicySchool of PharmacyUniversity of Southern California1985 Zonal Avenue, Los Angeles, CA, 90033, United States.
4
Southern California Research Center for Alcoholic Liver and Pancreatic Disease and Cirrhosis, Keck School of Medicine, University of Southern California, 1333 San Pablo Street, Los Angeles, CA 90033, United States.

Abstract

The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.

KEYWORDS:

Astrocytes; High fat diet; Intragastric ethanol feeding; Microglia; Neuroinflammation and neuronal loss; Pro-inflammatory cytokines; Purinergic P2X7 receptors

PMID:
26198936
PMCID:
PMC4512299
DOI:
10.1016/j.jneuroim.2015.06.007
[Indexed for MEDLINE]
Free PMC Article

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