Format

Send to

Choose Destination
PLoS Genet. 2015 Jul 21;11(7):e1005373. doi: 10.1371/journal.pgen.1005373. eCollection 2015 Jul.

Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress.

Author information

1
The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom.
2
Department of Plant Genetics, Breeding and Plant Biotechnology, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
3
Department of Biosciences, Plant Biology, University of Helsinki, Helsinki, Finland.
4
Laboratory of Biochemistry and Microspectroscopy Center, Wageningen University, Wageningen, The Netherlands.
5
Department of Plant and Environmental Sciences and Copenhagen Plant Science Center, University of Copenhagen, Frederiksberg, Denmark.
6
Institute of Technology, University of Tartu, Tartu, Estonia.
7
Department of Plant Genetics, Breeding and Plant Biotechnology, Warsaw University of Life Sciences-SGGW, Warsaw, Poland; Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, Warsaw, Poland.
8
Research Unit Environmental Simulation, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
9
Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
10
Department of Biosciences, Plant Biology, University of Helsinki, Helsinki, Finland; Institute of Technology, University of Tartu, Tartu, Estonia.
11
Department of Biosciences, Plant Biology, University of Helsinki, Helsinki, Finland; Distinguished Scientist Fellowship Program, College of Science, King Saud University, Riyadh, Saudi Arabia.

Abstract

Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains of unknown function 26 (DUF26) in their ectodomain. The CRKs form one of the largest groups of receptor-like protein kinases in plants, but their biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach of a nearly complete crk T-DNA insertion line collection showing that CRKs control important aspects of plant development and stress adaptation in response to biotic and abiotic stimuli in a non-redundant fashion. In particular, the analysis of reactive oxygen species (ROS)-related stress responses, such as regulation of the stomatal aperture, suggests that CRKs participate in ROS/redox signalling and sensing. CRKs play general and fine-tuning roles in the regulation of stomatal closure induced by microbial and abiotic cues. Despite their great number and high similarity, large-scale phenotyping identified specific functions in diverse processes for many CRKs and indicated that CRK2 and CRK5 play predominant roles in growth regulation and stress adaptation, respectively. As a whole, the CRKs contribute to specificity in ROS signalling. Individual CRKs control distinct responses in an antagonistic fashion suggesting future potential for using CRKs in genetic approaches to improve plant performance and stress tolerance.

PMID:
26197346
PMCID:
PMC4511522
DOI:
10.1371/journal.pgen.1005373
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center