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Mol Cells. 2015 Aug;38(8):697-704. doi: 10.14348/molcells.2015.0066. Epub 2015 Jul 21.

Dephosphorylation of DBC1 by Protein Phosphatase 4 Is Important for p53-Mediated Cellular Functions.

Author information

1
Department of Biological Sciences, College of Science, Chonnam National University, Gwangju 500-757, Korea.

Abstract

Deleted in breast cancer-1 (DBC1) contributes to the regulation of cell survival and apoptosis. Recent studies demonstrated that DBC is phosphorylated at Thr454 by ATM/ATR kinases in response to DNA damage, which is a critical event for p53 activation and apoptosis. However, how DBC1 phosphorylation is regulated has not been studied. Here we show that protein phosphatase 4 (PP4) dephosphorylates DBC1, regulating its role in DNA damage response. PP4R2, a regulatory subunit of PP4, mediates the interaction between DBC1 and PP4C, a catalytic subunit. PP4C efficiently dephosphorylates pThr454 on DBC1 in vitro, and the depletion of PP4C/PP4R2 in cells alters the kinetics of DBC1 phosphorylation and p53 activation, and increases apoptosis in response to DNA damage, which are compatible with the expression of the phosphomimetic DBC-1 mutant (T454E). These suggest that the PP4-mediated dephosphorylation of DBC1 is necessary for efficient damage responses in cells.

KEYWORDS:

DNA damage response; deleted in breast cancer-1; dephosphorylation; protein phosphatase 4

PMID:
26194823
PMCID:
PMC4546941
DOI:
10.14348/molcells.2015.0066
[Indexed for MEDLINE]
Free PMC Article

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