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Philos Trans R Soc Lond B Biol Sci. 2015 Sep 5;370(1676). pii: 20140237. doi: 10.1098/rstb.2014.0237.

Ageing of the B-cell repertoire.

Author information

1
Division of Infection, Immunity and Inflammatory Disease, King's College London Faculty of Life Sciences & Medicine, Guys Campus, London, UK.
2
Randall Division of Cell and Molecular Biophysics, King's College London Faculty of Life Sciences & Medicine, Guys Campus, London, UK.
3
Institute of Cancer and Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff, UK.
4
Division of Infection, Immunity and Inflammatory Disease, King's College London Faculty of Life Sciences & Medicine, Guys Campus, London, UK deborah.dunn-walters@kcl.ac.uk.

Abstract

Older people are more susceptible to infection, less responsive to vaccination and have a more inflammatory immune environment. Using spectratype analysis, we have previously shown that the B-cell repertoire of older people shows evidence of inappropriate clonal expansions in the absence of challenge, and that this loss of B-cell diversity correlates with poor health. Studies on response to vaccination, using both spectratyping and high-throughput sequencing of the repertoire, indicate that older responses to challenge are lacking in magnitude and/or delayed significantly. Also that some of the biologically significant differences may be in different classes of antibody. We have also previously shown that normal young B-cell repertoires can vary between different phenotypic subsets of B cells. In this paper, we present an analysis of immunoglobulin repertoire in different subclasses of antibody in five different populations of B cell, and show how the repertoire in these different groups changes with age. Although some age-related repertoire differences occur in naive cells, before exogenous antigen exposure, we see indications that there is a general dysregulation of the selective forces that shape memory B-cell populations in older people.

KEYWORDS:

B-cell memory; ageing; immunoglobulin repertoire; subclass of antibody

PMID:
26194751
PMCID:
PMC4528414
DOI:
10.1098/rstb.2014.0237
[Indexed for MEDLINE]
Free PMC Article

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