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Hemoglobin. 2015;39(5):371-4. doi: 10.3109/03630269.2015.1062775. Epub 2015 Jul 21.

Determination of Human Hemoglobin Derivatives.

Author information

1
a Biophysical Laboratory, Biochemistry Department , National Research Centre , Dokki , Cairo , Egypt.

Abstract

The levels of the inactive hemoglobin (Hb) pigments [such as methemoglobin (metHb), carboxyhemoglobin (HbCO) and sulfohemoglobin (SHb)] and the active Hb [in the oxyhemoglobin (oxyHb) form] as well as the blood Hb concentration in healthy non pregnant female volunteers were determined using a newly developed multi-component spectrophotometric method. The results of this method revealed values of SHb% in the range (0.0727-0.370%), metHb% (0.43-1.0%), HbCO% (0.4-1.52%) and oxyHb% (97.06-98.62%). Furthermore, the results of this method revealed values of blood Hb concentration in the range (12.608-15.777 g/dL). The method is highly sensitive, accurate and reproducible.

KEYWORDS:

Carboxyhemoglobin (HbCO); hemoglobin (Hb); humans; methemoglobin (metHb); oxyhemoglobin (oxyHb); sulfohemoglobin (SHb)

PMID:
26193973
DOI:
10.3109/03630269.2015.1062775
[Indexed for MEDLINE]

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