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Arch Gynecol Obstet. 1989;246(3):181-7.

Detection of early pregnancy factor (EPF) in pregnant and nonpregnant subjects with the rosette inhibition test.

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  • 1Klinik für Gynäkologie und Geburtshilfe, Ernst-Moritz-Arndt-Universität, Griefswald, GDR.


We tested for early pregnancy factor (EPF) using a rosette inhibition test with polyclonal anti-lymphocyte serum from the horse and two monoclonal antibodies specific for the E-receptor of T-lymphocytes. When lymphocytes were preincubated with early human pregnancy sera, rosette inhibition titres were four or more dilutions higher (P less than 0.01) than when lymphocytes were preincubated with nonpregnant sera.

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