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Nat Neurosci. 2015 Aug;18(8):1175-82. doi: 10.1038/nn.4065. Epub 2015 Jul 20.

Distinct brain transcriptome profiles in C9orf72-associated and sporadic ALS.

Author information

1
Department of Neuroscience, Mayo Clinic, Jacksonville, Florida, USA.
2
Information Technology, Mayo Clinic, Rochester, Minnesota, USA.
3
Department of Neurology, Mayo Clinic, Jacksonville, Florida, USA.
4
1] Department of Neuroscience, Mayo Clinic, Jacksonville, Florida, USA. [2] Mayo Graduate School, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.
5
Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota, USA.
6
Integrative Physiology, Institute for Behavioral Genetics, University of Colorado, Boulder, Colorado, USA.

Abstract

Increasing evidence suggests that defective RNA processing contributes to the development of amyotrophic lateral sclerosis (ALS). This may be especially true for ALS caused by a repeat expansion in C9orf72 (c9ALS), in which the accumulation of RNA foci and dipeptide-repeat proteins are expected to modify RNA metabolism. We report extensive alternative splicing (AS) and alternative polyadenylation (APA) defects in the cerebellum of c9ALS subjects (8,224 AS and 1,437 APA), including changes in ALS-associated genes (for example, ATXN2 and FUS), and in subjects with sporadic ALS (sALS; 2,229 AS and 716 APA). Furthermore, heterogeneous nuclear ribonucleoprotein H (hnRNPH) and other RNA-binding proteins are predicted to be potential regulators of cassette exon AS events in both c9ALS and sALS. Co-expression and gene-association network analyses of gene expression and AS data revealed divergent pathways associated with c9ALS and sALS.

PMID:
26192745
PMCID:
PMC4830686
DOI:
10.1038/nn.4065
[Indexed for MEDLINE]
Free PMC Article

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