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Int J Clin Exp Pathol. 2015 May 1;8(5):5217-23. eCollection 2015.

Antibacterial activity and mechanism of berberine against Streptococcus agalactiae.

Author information

1
College of Veterinary Medicine, Sichuan Agricultural University Ya'an, China.
2
College of Veterinary Medicine, Sichuan Agricultural University Ya'an, China ; Institute of Prevention Veterinary Medicine, Sichuan Agricultural University Chengdu, China.

Abstract

The antibacterial activity and mechanism of berberine against Streptococcus agalactiae were investigated in this study by analyzing the growth, morphology and protein of the S. agalactiae cells treated with berberine. The antibacterial susceptibility test result indicated minimum inhibition concentration (MIC) of berberine against Streptococcus agalactiae was 78 μg/mL and the time-kill curves showed the correlation of concentration-time. After the bacteria was exposed to 78 μg/mL berberine, the fragmentary cell membrane and cells unequal division were observed by the transmission electron microscopy (TEM), indicating the bacterial cells were severely damaged. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) study demonstrated that berberine could damage bacterial cells through destroying cellular proteins. Meanwhile, Fluorescence microscope revealed that berberine could affect the synthesis of DNA. In conclusion, these results strongly suggested that berberine may damage the structure of bacterial cell membrane and inhibit synthesis of protein and DNA, which cause Streptococcus agalactiae bacteria to die eventually.

KEYWORDS:

Berberine; SDS-PAGE; Streptococcus agalactiae; TEM; antibacterial activity

PMID:
26191220
PMCID:
PMC4503092
[Indexed for MEDLINE]
Free PMC Article

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