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Int J Clin Exp Pathol. 2015 May 1;8(5):5153-60. eCollection 2015.

Abnormal intestinal permeability and microbiota in patients with autoimmune hepatitis.

Author information

1
Department of Digestive Diseases, General Hospital, Tianjin Medical University Tianjin 300052, China.

Abstract

BACKGROUND:

Autoimmune hepatitis (AIH) is a chronic, progressive, and immunologically mediated inflammatory liver disorder. The etiology of AIH still remains unknown. The aim of this study was to investigate the changes in intestinal permeability, bacterial translocation, and intestinal microbiome in patients with AIH and to evaluate the correlations of those changes with the stages of the disease.

METHODS:

24 patients with autoimmune hepatitis and 8 healthy volunteers were recruited for this study. We assessed (1) the integrity of tight junctions within the gut by immunohistochemical analysis of zona occludens-1 and occludin expression in duodenal biopsy specimens; (2) changes in the enteric microbiome by 16S rDNA quantitative PCR; and (3) the presence of bacterial translocation by the level of lipopolysaccharide (LPS) using ELISA.

RESULTS:

Increased intestinal permeability, derangement of the microbiome and bacterial translocation occurred in AIH, which correlated with the severity of the disease.

CONCLUSIONS:

Autoimmune hepatitis is associated with leaky gut and intestinal microbiome dysbiosis. The impaired intestinal barrier may play an important role in the pathogenesis of AIH.

KEYWORDS:

Autoimmune hepatitis; bacterial; intestinal permeability; microbiota; translocation

PMID:
26191211
PMCID:
PMC4503083
[Indexed for MEDLINE]
Free PMC Article

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