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Cell Immunol. 2015 Oct;297(2):94-9. doi: 10.1016/j.cellimm.2015.07.003. Epub 2015 Jul 13.

Echinacea purpurea root extract inhibits TNF release in response to Pam3Csk4 in a phosphatidylinositol-3-kinase dependent manner.

Author information

1
Amway Corporation, 7575 Fulton St. East, Ada, MI 49355, USA. Electronic address: david.fast@amway.com.
2
Nutrilite Health Institute, 5600 Beach Boulevard, Buena Park, CA 90622, USA.
3
Amway Corporation, 7575 Fulton St. East, Ada, MI 49355, USA.

Abstract

Polysaccharides derived from Echinacea have historically been shown to be immunostimulatory. We describe in this work however the anti-inflammatory effect of a water extract of Echinacea purpurea roots (EPRW) that inhibited Pam3Csk4 stimulated production of TNFα by human monocytic THP-1 cells. The polyphenols and alkylamides typically found in Echinacea extracts were absent in EPRW suggesting that the anti-inflammatory component(s) was a polysaccharide. This anti-inflammatory activity was shown to be mediated by the phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway as chemical inhibition of PI3K abolished the EPRW anti-inflammatory effect. Demonstration of phosphorylation of Akt and ribosomal S6 proteins, downstream targets of PI3K confirmed EPRW-mediated activation of this pathway. In conclusion, this observation suggests that non-alkylamide/non-polyphenolic phytochemicals from Echinacea may contribute in part to some of the anti-inflammatory therapeutic effects such as reduced severity of symptoms that have been observed in vivo in the treatment of upper respiratory tract infections with Echinacea.

KEYWORDS:

Akt; Echinacea; PI3K; Pam(3)Csk(4); Polysaccharide; THP-1

PMID:
26190752
DOI:
10.1016/j.cellimm.2015.07.003
[Indexed for MEDLINE]

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