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Int J Radiat Biol. 2015;91(11):898-907. doi: 10.3109/09553002.2015.1075075.

Eight hours of nocturnal 915 MHz radiofrequency identification (RFID) exposure reduces urinary levels of melatonin and its metabolite via pineal arylalkylamine N-acetyltransferase activity in male rats.

Author information

1
a Department of Neurosurgery , Ajou University School of Medicine , Suwon ;
2
b College of Pharmacy , Sunchon National University , Sunchon ;
3
c Neuroscience Graduate Program, Department of Biomedical Sciences , Graduate School of Ajou University , Suwon ;
4
d Division of Life Science and Pharmaceuticals, College of Pharmacy , Ewha Woman's University , Seoul ;
5
e Radio Technology Research Department , Electronics and Telecommunications Research Institute , Daejeon ;
6
f Department of Radio Sciences and Engineering, College of Engineering , Chungnam National University , Daejeon ;
7
g School of Electrical and Computer Engineering , Chungbuk National University , Cheongju , Republic of Korea.

Abstract

PURPOSE:

We investigated the effects of whole-body exposure to the 915 MHz radiofrequency identification (RFID) on melatonin biosynthesis and the activity of rat pineal arylalkylamine N-acetyltransferase (AANAT).

MATERIALS AND METHODS:

Rats were exposed to RFID (whole-body specific absorption rate, 4 W/kg) for 8 h/day, 5 days/week, for weeks during the nighttime. Total volume of urine excreted during a 24-h period was collected after RFID exposure. Urinary melatonin and 6-hydroxymelatonin sulfate (6-OHMS) was measured by gas chromatography-mass spectrometry (GC-MS) and enzyme-linked immunosorbent assay (ELISA), respectively. AANAT enzyme activity was measured using liquid biphasic dif-13 fusion assay. Protein levels and mRNA expression of AANAT was 14 measured by Western blot and reverse transcription polymerase 15 chain reaction (RT-PCR) analysis, respectively.

RESULTS:

Eight hours of nocturnal RFID exposure caused a significant reduction in both urinary melatonin (p = 0. 003) and 6-OHMS (p = 0. 026). Activity, protein levels, and mRNA expression of AANAT were suppressed by exposure to RFID (p < 0. 05).

CONCLUSIONS:

Our results suggest that nocturnal RFID exposure can cause reductions in the levels of both urinary melatonin and 6-OHMS, possibly due to decreased melatonin biosynthesis via suppression of Aanat gene transcription in the rat pineal gland.

KEYWORDS:

Radiofrequency identification; melatonin; pineal gland; rat

PMID:
26189731
DOI:
10.3109/09553002.2015.1075075
[Indexed for MEDLINE]

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