Format

Send to

Choose Destination
Aging (Albany NY). 2015 Jul;7(7):475-85.

Mitochondria-targeted antioxidant SkQ1 improves impaired dermal wound healing in old mice.

Author information

1
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
2
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
3
Institute of Mitoengineering, Lomonosov Moscow State University, Moscow, Russia.
4
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.

Abstract

The process of skin wound healing is delayed or impaired in aging animals. To investigate the possible role of mitochondrial reactive oxygen species (mtROS) in cutaneous wound healing of aged mice, we have applied the mitochondria-targeted antioxidant SkQ1. The SkQ1 treatment resulted in accelerated resolution of the inflammatory phase, formation of granulation tissue, vascularization and epithelization of the wounds. The wounds of SkQ1-treated mice contained increased amount of myofibroblasts which produce extracellular matrix proteins and growth factors mediating granulation tissue formation. This effect resembled SkQ1-induced differentiation of fibroblasts to myofibroblast, observed earlierin vitro. The Transforming Growth Factor beta (TGFb) produced by SkQ1-treated fibroblasts was found to stimulated motility of endothelial cells in vitro, an effect which may underlie pro-angiogenic action of SkQ1 in the wounds. In vitro experiments showed that SkQ1 prevented decomposition of VE-cadherin containing contacts and following increase in permeability of endothelial cells monolayer, induced by pro-inflammatory cytokine TNF. Prevention of excessive reaction of endothelium to the pro-inflammatory cytokine(s) might account for anti-inflammatory effect of SkQ1. Our findings point to an important role of mtROS in pathogenesis of age-related chronic wounds.

KEYWORDS:

mitochondria-targeted antioxidant; mitochondrial reactive oxygen species; wound healing

PMID:
26187706
PMCID:
PMC4543037
DOI:
10.18632/aging.100772
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Impact Journals, LLC Icon for PubMed Central
Loading ...
Support Center