Format

Send to

Choose Destination
Cell. 2015 Jul 16;162(2):246-257. doi: 10.1016/j.cell.2015.06.067.

Clarifying Tissue Clearing.

Author information

1
Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: drichardson@fas.harvard.edu.
2
Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Center for Brain Science, Harvard University, Cambridge, MA 02138, USA. Electronic address: jeff@mcb.harvard.edu.

Abstract

Biological specimens are intrinsically three dimensional; however, because of the obscuring effects of light scatter, imaging deep into a tissue volume is problematic. Although efforts to eliminate the scatter by "clearing" the tissue have been ongoing for over a century, there have been a large number of recent innovations. This Review introduces the physical basis for light scatter in tissue, describes the mechanisms underlying various clearing techniques, and discusses several of the major advances in light microscopy for imaging cleared tissue.

PMID:
26186186
PMCID:
PMC4537058
DOI:
10.1016/j.cell.2015.06.067
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center