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Genome Biol Evol. 2015 Jul 15;7(8):2154-72. doi: 10.1093/gbe/evv131.

Comparative Genomics of Listeria Sensu Lato: Genus-Wide Differences in Evolutionary Dynamics and the Progressive Gain of Complex, Potentially Pathogenicity-Related Traits through Lateral Gene Transfer.

Author information

1
Dipartimento di Bioscienze, Università degli Studi di Milano, Italy Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy.
2
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy.
3
Dipartimento di Bioscienze, Biotecnologie e Biofarmaceutica, Università degli Studi di Bari Aldo Moro, Italy Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Bari, Italy.
4
Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Bari, Italy.
5
Dipartimento di Bioscienze, Università degli Studi di Milano, Italy david.horner@unimi.it parisi.izspb@gmail.com.
6
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy david.horner@unimi.it parisi.izspb@gmail.com.

Abstract

Historically, genome-wide and molecular characterization of the genus Listeria has concentrated on the important human pathogen Listeria monocytogenes and a small number of closely related species, together termed Listeria sensu strictu. More recently, a number of genome sequences for more basal, and nonpathogenic, members of the Listeria genus have become available, facilitating a wider perspective on the evolution of pathogenicity and genome level evolutionary dynamics within the entire genus (termed Listeria sensu lato). Here, we have sequenced the genomes of additional Listeria fleischmannii and Listeria newyorkensis isolates and explored the dynamics of genome evolution in Listeria sensu lato. Our analyses suggest that acquisition of genetic material through gene duplication and divergence as well as through lateral gene transfer (mostly from outside Listeria) is widespread throughout the genus. Novel genetic material is apparently subject to rapid turnover. Multiple lines of evidence point to significant differences in evolutionary dynamics between the most basal Listeria subclade and all other congeners, including both sensu strictu and other sensu lato isolates. Strikingly, these differences are likely attributable to stochastic, population-level processes and contribute to observed variation in genome size across the genus. Notably, our analyses indicate that the common ancestor of Listeria sensu lato lacked flagella, which were acquired by lateral gene transfer by a common ancestor of Listeria grayi and Listeria sensu strictu, whereas a recently functionally characterized pathogenicity island, responsible for the capacity to produce cobalamin and utilize ethanolamine/propane-2-diol, was acquired in an ancestor of Listeria sensu strictu.

KEYWORDS:

Listeria; cobalamin; comparative genomics; ethanolamine metabolism; flagella; genome sequencing; lateral gene transfer; propane-2-diol metabolism

PMID:
26185097
PMCID:
PMC4558849
DOI:
10.1093/gbe/evv131
[Indexed for MEDLINE]
Free PMC Article

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