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Macromol Biosci. 2015 Nov;15(11):1586-94. doi: 10.1002/mabi.201500115. Epub 2015 Jul 17.

Neurogenesis Is Induced by Electrical Stimulation of Human Mesenchymal Stem Cells Co-Cultured With Mature Neuronal Cells.

Author information

1
Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul-si, 139-240, Korea.
2
Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-Complex, 689 Sampyeong-dong Bundang-gu, Seongnam-si, 463-400, Korea.
3
Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 215-4, Gongneung, Nowon, Seoul-si, 139-240, Korea. chkim@kirams.re.kr.
4
Department of Biomedical Science, College of Life Science, CHA University, 6F, CHA Bio-Complex, 689 Sampyeong-dong Bundang-gu, Seongnam-si, 463-400, Korea. pkh0410@cha.ac.kr.

Abstract

For electrical stimulation of hMSCs, gold nanoparticles were coated onto polyethyleneimine coated glass cover slips. The effects of pulsed or constant electrical stimulation upon cytotoxicity and differentiation of hMSCs were examined. The effects of co culturing hMSCs with neuronal cells were also tested. The neuronal differentiation of the stem cells was evaluated by determining the expression of neuron-specific genes and proteins using RT-PCR and Western blotting. Morphological changes were evaluated by scanning electron microscopy. The hMSCs co-cultured with mature neuronal cells and stimulated with electrical shock showed the greatest level of neurite outgrowth (>150 mm) and smaller cell body sizes.

KEYWORDS:

differentiation; electrical stimulation; gold nanoparticles; hMSCs; nerve cell

PMID:
26183918
DOI:
10.1002/mabi.201500115
[Indexed for MEDLINE]

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