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Nat Commun. 2015 Jul 17;6:7814. doi: 10.1038/ncomms8814.

Insect glycerol transporters evolved by functional co-option and gene replacement.

Author information

1
1] Department of Biology, Bergen High Technology Center, University of Bergen, PO Box 7803, N-5020 Bergen, Norway [2] Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway.
2
1] Department of Biology, Bergen High Technology Center, University of Bergen, PO Box 7803, N-5020 Bergen, Norway [2] Institut de Recerca i Tecnologia Agroalimentàries (IRTA)-Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), Passeig Marítim 37-49, 08003 Barcelona, Spain.
3
Department of Biology, Bergen High Technology Center, University of Bergen, PO Box 7803, N-5020 Bergen, Norway.
4
Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Passeig Marítim 37-49, 08003 Barcelona, Spain.
5
Institut de Recerca i Tecnologia Agroalimentàries (IRTA)-Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), Passeig Marítim 37-49, 08003 Barcelona, Spain.

Abstract

Transmembrane glycerol transport is typically facilitated by aquaglyceroporins in Prokaryota and Eukaryota. In holometabolan insects however, aquaglyceroporins are absent, yet several species possess polyol permeable aquaporins. It thus remains unknown how glycerol transport evolved in the Holometabola. By combining phylogenetic and functional studies, here we show that a more efficient form of glycerol transporter related to the water-selective channel AQP4 specifically evolved and multiplied in the insect lineage, resulting in the replacement of the ancestral branch of aquaglyceroporins in holometabolan insects. To recapitulate this evolutionary process, we generate specific mutants in distantly related insect aquaporins and human AQP4 and show that a single mutation in the selectivity filter converted a water-selective channel into a glycerol transporter at the root of the crown clade of hexapod insects. Integration of phanerozoic climate models suggests that these events were associated with the emergence of complete metamorphosis and the unparalleled radiation of insects.

PMID:
26183829
PMCID:
PMC4518291
DOI:
10.1038/ncomms8814
[Indexed for MEDLINE]
Free PMC Article

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