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Neuron. 2015 Jul 15;87(2):326-40. doi: 10.1016/j.neuron.2015.06.028.

Single-Cell mRNA Profiling Reveals Cell-Type-Specific Expression of Neurexin Isoforms.

Author information

1
Department of Molecular and Cellular Physiology, School of Medicine, Stanford University, Stanford, CA 94305, USA; Nancy Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, School of Medicine, Stanford University, Stanford, CA 94305, USA. Electronic address: fuccillo@mail.med.upenn.edu.
2
Department of Molecular and Cellular Physiology, School of Medicine, Stanford University, Stanford, CA 94305, USA; Nancy Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, School of Medicine, Stanford University, Stanford, CA 94305, USA.
3
Department of Molecular and Cellular Physiology, School of Medicine, Stanford University, Stanford, CA 94305, USA.
4
Nancy Pritzker Laboratory, Department of Psychiatry and Behavioral Sciences, School of Medicine, Stanford University, Stanford, CA 94305, USA.
5
Department of Molecular and Cellular Physiology, School of Medicine, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, School of Medicine, Stanford University, Stanford, CA 94305, USA. Electronic address: tcs1@stanford.edu.

Abstract

Neurexins are considered central organizers of synapse architecture that are implicated in neuropsychiatric disorders. Expression of neurexins in hundreds of alternatively spliced isoforms suggested that individual neurons might exhibit a cell-type-specific neurexin expression pattern (a neurexin code). To test this hypothesis, we quantified the single-cell levels of neurexin isoforms and other trans-synaptic cell-adhesion molecules by microfluidics-based RT-PCR. We show that the neurexin repertoire displays pronounced cell-type specificity that is remarkably consistent within each type of neuron. Furthermore, we uncovered region-specific regulation of neurexin transcription and splice-site usage. Finally, we demonstrate that the transcriptional profiles of neurexins can be altered in an experience-dependent fashion by exposure to a drug of abuse. Our data provide evidence of cell-type-specific expression patterns of multiple neurexins at the single-cell level and suggest that expression of synaptic cell-adhesion molecules overlaps with other key features of cellular identity and diversity.

PMID:
26182417
PMCID:
PMC4733560
DOI:
10.1016/j.neuron.2015.06.028
[Indexed for MEDLINE]
Free PMC Article

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