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Oxid Med Cell Longev. 2015;2015:154164. doi: 10.1155/2015/154164. Epub 2015 Jun 9.

Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Author information

1
Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.
2
Merck Millipore Bioscience Division, 78180 Saint-Quentin-en-Yvelines, France.
3
Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France ; Department of Medical Chemistry and Biochemistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
4
Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France ; UMR 7200 CNRS, Faculté de Pharmacie, 75 route du Rhin, 67400 Illkirch, France.

Abstract

SCOPE:

The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

METHODS AND RESULTS:

Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

CONCLUSION:

We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

PMID:
26180579
PMCID:
PMC4477188
DOI:
10.1155/2015/154164
[Indexed for MEDLINE]
Free PMC Article

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