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Haemophilia. 2015 Sep;21(5):e402-10. doi: 10.1111/hae.12743. Epub 2015 Jul 14.

Longitudinal assessment of bone loss using quantitative ultrasound in a blood-induced arthritis rabbit model.

Author information

1
Department of Diagnostic Imaging, The Hospital for Sick Children, Toronto, ON, Canada.
2
Department of Family and Community Medicine, University of Toronto, Toronto, ON, Canada.
3
Department of Pathology, Mount Sinai Hospital, Toronto, ON, Canada.
4
Department of Neonatology, The Hospital for Sick Children, Toronto, ON, Canada.

Abstract

INTRODUCTION:

Osteoporosis is common in haemophilic arthropathy. Quantitative ultrasound (QUS) can be a suitable alternative for dual-energy x-ray absorptiometry for diagnosing osteoporosis in haemophiliacs due to its lack of ionizing radiation, and ease to use.

AIM:

We investigated the intra- and inter-operator reliability of QUS, its responsiveness to bone growth, its ability to differentiate bone adjacent to blood-injected vs. control joints, and the effect of soft tissues on the speed of sound (SOS) QUS values in a juvenile white New Zealand rabbit model of blood-induced arthritis.

METHODS:

Eight of 16 rabbits were injected with autologous blood (0.1 mL kg(-1) ) 8 times over a 17-week period, the remaining eight rabbits served as controls. SOS was measured at baseline, weeks 8 and 17 in vivo and after the bones were excised on week 17.

RESULTS:

Intra- and inter-operator coefficients of variation for QUS data were <5% and intraclass correlation coefficients were >60% for 22/27 (81.5%) of bones assessed. The level of interval increase in SOS values from baseline to week 17 was significantly different in tibiae of injected, contralateral to injected and non-injected knee groups by anova (P = 0.01). In vivo (mean ± SD, 4147.17 ± 96.27 m s(-1) ) and postmortem (4457.85 ± 104.00 m s(-1) ) measurements on week 17 differed (P < 0.01) indicating an effect of soft tissues on SOS.

CONCLUSION:

In conclusion, QUS' acceptable reliability, its responsiveness to growth-related changes and its ability to discriminate injected and non-injected joints make this technique a plausible candidate as a diagnostic tool for osteoporosis in the paediatric haemophilic population if these results are confirmed upon animal-human translation.

KEYWORDS:

blood-induced arthritis model; haemophilia; knees; osteoporosis; quantitative ultrasound; rabbit

PMID:
26178807
DOI:
10.1111/hae.12743
[Indexed for MEDLINE]

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