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Inflammation. 2015 Dec;38(6):2269-78. doi: 10.1007/s10753-015-0212-3.

Observing Anti-inflammatory and Anti-nociceptive Activities of Glycyrrhizin Through Regulating COX-2 and Pro-inflammatory Cytokines Expressions in Mice.

Author information

1
Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, 750004, China.
2
College of Nursing, Ningxia Medical University, Yinchuan, 750004, China.
3
Laboratory Animal Center, Ningxia Medical University, Yinchuan, 750004, China.
4
Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, 750004, China.
5
Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China.
6
Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, 750004, China. Yujq910315@163.com.
7
Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Yinchuan, 750004, China. Yujq910315@163.com.

Abstract

The present study aimed to investigate the potential anti-inflammatory and anti-nociceptive activities of glycyrrhizin (GL) in mice and to explore the possible related mechanisms. Xylene-induced ear edema, carrageenan-induced paw edema and acetic acid-induced vascular permeability test were used to investigate the anti-inflammatory activities of GL in mice. Anti-nociceptive effects of GL were assessed by using acetic acid-induced writhing, hot plate test and formalin test, as well as evaluation of spontaneous locomotor activity and motor performance. The mRNA expression of pro-inflammatory cytokines (such as TNF-α, IL-6 and iNOS) and the protein expression of cyclooxygenase-2 (COX-2) were explored by using real-time fluorogenic PCR and Western blot, respectively. The results showed that GL significantly reduced xylene-induced ear edema, carrageenan-induced paw edema, and acetic acid-induced vascular permeation. Additionally, GL significantly inhibited the nociceptions induced by acetic acid and formalin. However, the nociceptions could not be decreased by GL in the hot plate test, and GL did not affect spontaneous locomotor activity and motor performance. The expression levels of TNF-α, IL-6, iNOS and COX-2 were significantly downregulated by GL. In conclusion, GL exerts significant anti-inflammatory and analgesic activities by attenuating the expression levels of TNF-α, IL-6, iNOS and COX-2.

KEYWORDS:

anti-inflammatory activity; anti-nociceptive activity; cyclooxygenase-2; glycyrrhizin; pro-inflammatory cytokines

PMID:
26178479
DOI:
10.1007/s10753-015-0212-3
[Indexed for MEDLINE]

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