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Curr Eye Res. 2016 May;41(5):721-4. doi: 10.3109/02713683.2015.1068816. Epub 2015 Jul 15.

Short Communication: Increase of Sialylated N-Glycansin Eyes with Neovascular Glaucoma Secondary to Proliferative Diabetic Retinopathy.

Author information

1
a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan .
2
b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan .
3
c Field of Drug Discovery Research, Faculty of Advanced Life Science, Graduate School of Life Science, Hokkaido University , Sapporo , Japan and.
4
d Medicinal Chemistry Pharmaceuticals, Co. Ltd. , Sapporo , Japan.

Abstract

PURPOSE:

To investigate the alteration of N-glycans in the vitreous fluid of patients with neovascular glaucoma (NVG) secondary to proliferative diabetic retinopathy (PDR).

METHODS:

Vitreous samples were collected from 18 patients with PDR (including 7 with NVG and 11 without NVG), and 17 patients without diabetes. Profiles of N-glycans were analyzed by glycoblotting-based high throughput protocol, which we recently developed. Protein levels of vascular endothelial growth factor (VEGF)-A were measured by ELISA.

RESULTS:

The concentration of total N-glycans and the concentration of N-glycans with sialic acids were significantly higher in NVG group compared with those in non-NVG group or control group, whereas there was no significant difference in concentrations of high-mannose N-glycans among three groups. There was a moderate correlation between the concentrations of sialylated N-glycans and VEGF-A.

CONCLUSIONS:

Our data demonstrate the distinct changes of N-glycan profile and the increase of sialylated N-glycans in eyes with NVG secondary to PDR.

KEYWORDS:

N-glycans; neovascular glaucoma; proliferative diabetic retinopathy

PMID:
26177396
DOI:
10.3109/02713683.2015.1068816
[Indexed for MEDLINE]

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