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Curr Opin Chem Biol. 2015 Oct;28:105-14. doi: 10.1016/j.cbpa.2015.06.026. Epub 2015 Jul 10.

Advances in de novo strain design using integrated systems and synthetic biology tools.

Author information

1
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA.
2
Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA. Electronic address: costas@psu.edu.

Abstract

Recent efforts in expanding the range of biofuel and biorenewable molecules using microbial production hosts have focused on the introduction of non-native pathways in model organisms and the bio-prospecting of non-model organisms with desirable features. Current challenges lie in the assembly and coordinated expression of the (non-)native pathways and the elimination of competing pathways and undesirable regulation. Several systems and synthetic biology approaches providing contrasting top-down and bottom-up strategies, respectively, have been developed. In this review, we discuss recent advances in both in silico and experimental approaches for metabolic pathway design and engineering, with a critical assessment of their merits and remaining challenges.

PMID:
26177080
DOI:
10.1016/j.cbpa.2015.06.026
[Indexed for MEDLINE]

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