Format

Send to

Choose Destination
PLoS One. 2015 Jul 15;10(7):e0133053. doi: 10.1371/journal.pone.0133053. eCollection 2015.

Up in Arms: Immune and Nervous System Response to Sea Star Wasting Disease.

Author information

1
Department of Biology, University of Texas at Arlington, Arlington, Texas, United States of America.
2
Department of Ecology & Evolutionary Biology, Cornell University, Ithaca, New York, United States of America.
3
Department of Biology, Pennsylvania State University, University Park, Pennsylvania, United States of America.
4
Donald P. Shiley Bioscience Center, San Diego, California, United States of America.
5
Marine Science Center, Northeastern University, Nahant, Massachusetts, United States of America.
6
Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, California, United States of America.
7
Department of Ecology & Evolutionary Biology, Cornell University, Ithaca, New York, United States of America; School of Aquatic & Fishery Sciences, University of Washington, Seattle, Washington, United States of America.
8
School of Aquatic & Fishery Sciences, University of Washington, Seattle, Washington, United States of America.
9
Department of Microbiology, Cornell University, Ithaca, New York, United States of America.
10
U. S. Geological Survey, Western Fisheries Research Center, Marrowstone Marine Field Station, Nordland, Washington, United States of America.

Abstract

Echinoderms, positioned taxonomically at the base of deuterostomes, provide an important system for the study of the evolution of the immune system. However, there is little known about the cellular components and genes associated with echinoderm immunity. The 2013-2014 sea star wasting disease outbreak is an emergent, rapidly spreading disease, which has led to large population declines of asteroids in the North American Pacific. While evidence suggests that the signs of this disease, twisting arms and lesions, may be attributed to a viral infection, the host response to infection is still poorly understood. In order to examine transcriptional responses of the sea star Pycnopodia helianthoides to sea star wasting disease, we injected a viral sized fraction (0.2 μm) homogenate prepared from symptomatic P. helianthoides into apparently healthy stars. Nine days following injection, when all stars were displaying signs of the disease, specimens were sacrificed and coelomocytes were extracted for RNA-seq analyses. A number of immune genes, including those involved in Toll signaling pathways, complement cascade, melanization response, and arachidonic acid metabolism, were differentially expressed. Furthermore, genes involved in nervous system processes and tissue remodeling were also differentially expressed, pointing to transcriptional changes underlying the signs of sea star wasting disease. The genomic resources presented here not only increase understanding of host response to sea star wasting disease, but also provide greater insight into the mechanisms underlying immune function in echinoderms.

PMID:
26176852
PMCID:
PMC4503460
DOI:
10.1371/journal.pone.0133053
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center