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Evodevo. 2015 May 1;6:17. doi: 10.1186/s13227-015-0009-3. eCollection 2015.

Wnt affects symmetry and morphogenesis during post-embryonic development in colonial chordates.

Author information

1
School of Bioscience, University of Birmingham, Edgbaston, Birmingham, B19 2TT UK.
2
Molecular Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA 93106 USA.
3
CNRS, Sorbonne Universités, UPMC Univ Paris 06, Laboratoire de Biologie du Développement de Villefranche-sur-mer, Observatoire Océanographique, 06230 Villefranche-sur-mer, France.
#
Contributed equally

Abstract

BACKGROUND:

Wnt signaling is one of the earliest and most highly conserved regulatory pathways for the establishment of the body axes during regeneration and early development. In regeneration, body axes determination occurs independently of tissue rearrangement and early developmental cues. Modulation of the Wnt signaling in either process has shown to result in unusual body axis phenotypes. Botryllus schlosseri is a colonial ascidian that can regenerate its entire body through asexual budding. This processes leads to an adult body via a stereotypical developmental pathway (called blastogenesis), without proceeding through any embryonic developmental stages.

RESULTS:

In this study, we describe the role of the canonical Wnt pathway during the early stages of asexual development. We characterized expression of three Wnt ligands (Wnt2B, Wnt5A, and Wnt9A) by in situ hybridization and qRT-PCR. Chemical manipulation of the pathway resulted in atypical budding due to the duplication of the A/P axes, supernumerary budding, and loss of the overall cell apical-basal polarity.

CONCLUSIONS:

Our results suggest that Wnt signaling is used for equivalent developmental processes both during embryogenesis and asexual development in an adult organism, suggesting that patterning mechanisms driving morphogenesis are conserved, independent of embryonic, or regenerative development.

KEYWORDS:

Asexual development; Chordates; Polarity; Symmetry; Wnt

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